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- #!/usr/bin/env python
- # -*- coding: UTF-8 -*-
- #
- # Copyright (C) 2016 Paul Müller (paul.mueller [at] biotec.tu-dresden.de)
- #
- # This file is part of scattnlay
- #
- # This program is free software: you can redistribute it and/or modify
- # it under the terms of the GNU General Public License as published by
- # the Free Software Foundation, either version 3 of the License, or
- # (at your option) any later version.
- #
- # This program is distributed in the hope that it will be useful,
- # but WITHOUT ANY WARRANTY; without even the implied warranty of
- # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
- # GNU General Public License for more details.
- #
- # The only additional remark is that we expect that all publications
- # describing work using this software, or all commercial products
- # using it, cite the following reference:
- # [1] O. Pena and U. Pal, "Scattering of electromagnetic radiation by
- # a multilayered sphere," Computer Physics Communications,
- # vol. 180, Nov. 2009, pp. 2348-2354.
- #
- # You should have received a copy of the GNU General Public License
- # along with this program. If not, see <http://www.gnu.org/licenses/>.
- # This test case calculates the phase retardation that is introduced
- # by a weak dielectric sphere to an incident plane wave. Only the
- # x-polarized light is considered.
- # Note: This example computes the phase behind the sphere. In microscopy,
- # the focal plane during imaging is close to the center of the sphere.
- # To compute the phase image that corresponds to that imaged with a focal
- # plane at the center of the bead, numerical refocusing of the computed
- # field `Ex` would be required (e.g. python package nrefocus).
- import numpy as np
- import scattnlay
- import matplotlib.pylab as plt
- # weak dielectric sphere, e.g. a PMMA gel bead
- n1 = 1.335
- # refractive index of the surrounding medium (water)
- nm = 1.333
- # radius of the sphere in vacuum wavelengths
- radius = 0.3
- # extent of the simulation size in vacuum wavelengths
- extent = 2.0
- # distance where we want to have the measured field behind the sphere
- # in vacuum wavelengths measured from the center of the sphere
- distance = 0.5
- # pixels per vacuum wavelength in the output image
- resolution = 20.0
- # size parameters need to be multiplied by (2 PI nm) for the computation
- twopi = 2*np.pi*nm
- # There is only one sphere, no layers
- x = np.array([radius*twopi], dtype = np.float64)
- # Set the refractive index of the sphere, normalized to that of the medium
- m = np.array([n1/nm], dtype = np.complex128)
- nptsx = int(extent*resolution)
- nptsy = int(extent*resolution)
- scanx = np.linspace(-extent/2, extent/2, nptsx, endpoint=True)*twopi
- scany = np.linspace(-extent/2, extent/2, nptsy, endpoint=True)*twopi
- coordX, coordY = np.meshgrid(scanx, scany)
- coordX.resize(nptsx*nptsy)
- coordY.resize(nptsx*nptsy)
- coordZ = np.ones(nptsx*nptsy, dtype=np.float64)*distance*twopi
- terms, E, H = scattnlay.fieldnlay(x, m, coordX, coordY, coordZ)
- # take the x-component of the electric field
- Ex = E[:,0].reshape(nptsx, nptsy)
- # normalize by the background field (free space propagation)
- Ex /= np.exp(2j*np.pi*distance*nm)
- # plot the phase (np.angle) of the x-component of the electric field
- ax = plt.subplot(111)
- mapper = plt.imshow(np.angle(Ex))
- plt.colorbar(mapper, ax=ax, label="phase [rad]")
- plt.title("phase retardation introduced by a dielectric sphere")
- plt.show()
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